Studies on the migration of mouse germ cells.

P-48: Nicotine Alters Both Somatic and Germ Cells in Adult Mouse Testis

Background: Nicotine as a toxic agent in cigarette has detrimental effects on reproduction. The aim of this study was to evaluate effects of of nicotine on germ and somatic cells in adult mouse testis. Materials and Methods: Male mice were divided into four groups. Group A or controls, groups of B, C and D were treated with nicotine intraperitoneally in doses of 0.1, 0.2 and 0.4 mg/100 g body w...

I-18: Avian Chimeras and Germ Cell Migration

Background: In avian species, the germ line stem cell population arises outside of the embryonic gonad and proceeds on a circuitous migration to the germinal epithelium. Specifically, in the avian embryo, the process of germ line stem cell migration proceeds through a series of active and passive migratory phases. The germline stem cells or primordial germ cells (PGCs) located in the epiblast o...

The effect of BMP4 on mouse embryonic stem cell proliferation and differentiation into primordial germ cells

Background and Aim: Artificial gamete production from stem cells is a novel strategy for treatment of infertility. Among various stem cell sources, embryonic stem cells (ESC) can be considered as an appropriate source for in vitro formation of germ cells. In this study we evaluated the effect of BMP4 on proliferation and differentiation of mouse embryonic stem cells into primordial germ cells (...

Co-culture of Mouse Embryonic Stem Cells with Sertoli Cells Promote in vitro Generation of Germ Cells

  Objective(s): Sertoli cells support in vivo germ cell production; but, its exact mechanism has not been well understood. The present study was designed to analyze the effect of Sertoli cells in differentiation of mouse embryonic stem cells (mESCs) to germ cells.   Materials and Methods: A fusion construct composed of a Stra8 gene promoter and the coding region of enhanced green fluorescence p...

Functional Concentrations of BMP4 on Differentiation of Mouse Embryonic Stem Cells to Primordial Germ Cells

P-10: Proliferative Activity of Adult Mouse Male Germ Cells Following Administration of Graded Doses of Nicotine

Background: Nicotine is considered as one of the most agents in cigarette smoking and has side effects on many organs and body systems such as reproductive tract. Nicotine can induce sub fertility or infertility both in males and females. The aim of this study was to evaluate the proliferative activity of adult mouse male germ cells following treatment with different doses of nicotine. Material...